IMMUNOCHEMICAL CHARACTERISATION OF SOYABEAN GLYCININ FRAGMENTATION ESCAPING SMALL INTESTINAL DIGESTION IN PRE-RUMINANT CALVES.

Abstract

Attempts were made to precise some biochemical features of immune reactive fragments of glycine previously evidenced by ELISA in ideal digest of calves fed a milk replacer containing antigenic soya bean. This was carried out by a panel of specific polyclonal antisera and monoclonal antibodies in immunoblotting after SDS-PAGE. Under reducing conditions, two major polypeptides of mar 17,000 and 21,000 were observed, the latter corresponding to undigested basic (B) polypeptides of glycine. Putative acidic (A) fragments were not directly evidenced under reducing conditions. Under non-reducing conditions, four bands were observed
with mar of around 28, 000, 35, 000, 38,000 and 42,000. The two lighter bands were identified as partially digested AB-type structures. Assuming B polypeptides to be rather intact (mar 21,000), undigested A fragments in the digest were estimated to have mar of around 7, 000, 14, 000, .17, 000 and 21,000. It was concluded that large fragments of glycine escaped small intestinal digestion in the calf, and that structures of AB type and possibly quaternary associations were still present.