Comparative Analysis of the Dark Ground Buffy Coat Technique (DG) and Enzyme-Linked Immunosorbent Assay (ELISA) in the Diagnosis of Trypanosomosis in Cattle

Abstract

The prevalence of typanosome i~lfectioinn 65catte reared under expensive system of inanagement
was determined using the dark ground buffy coat (DG) tecl?nique and tile enzyme-linked
i~~;niunosorbaets s@ LISA). TZ?e DG technique showed that there were 28positive cases (27.69%)
of totalnumber ofa nimaIs, made up of6 of Try~~anosomcoan golense, i'of 7: Mvaxand3of T. Brucei. +
Other cases were tl~oseo f mixed infectionsm ai17ly one case of which all of 3 Tryyansomes species
were present, and another in which I: vivax, 32 of T bruceiand 2 cases of 7: brucei were 30, (46.15%
positive casesmacfe up of 6 of I: congoIense, 7of T. vivax, l2of I: bruceiand2 cases of I: brucie mixed
with 7: Congiolese. Tl~EeL ISA teclmique did not detect any incidence ofm ixed infectionsi n wl~icl?
the 3 species of trypanosomes were present.